TurFresh Enzyme Cleaner: How to Use BioS+ for Maximum Odor Control

Quick Answer:
TurFresh enzyme cleaner BioS+ works by using bacterial enzyme technology to break down uric acid, ammonia, and organic waste at a molecular level rather than masking the odor. The correct mix ratio for the concentrate is 3.2 oz of BioS+ Super Concentrate to 28.2 oz of water to produce one 32 oz ready-to-use bottle. Apply when the turf surface is cool and out of direct sunlight, allow a minimum of 10 minutes of dwell time before rinsing or allowing foot traffic, and rinse the area with water first to help activate the enzymes. One quart of ready-to-use solution treats up to 500 square feet of artificial grass. The two most common reasons BioS+ does not produce expected results are applying to hot turf in direct sun and rinsing immediately after application before dwell time completes.

 

 

Key Takeaways

✅ The correct mix ratio for BioS+ Super Concentrate is 3.2 oz concentrate to 28.2 oz water for a 32 oz bottle. Using too much concentrate does not improve results and wastes product. Using too little reduces enzyme concentration below effective levels. Mix only what you plan to use immediately as diluted enzyme solution loses effectiveness within hours.

✅ Dwell time is the step most users skip, and it is why results disappoint. Enzymes need a minimum of 10 minutes of contact time to break down uric acid crystals and organic waste. Rinsing immediately after application stops the process before it completes. Set a timer.

✅ Never apply to hot turf in direct sunlight. Heat deactivates enzymes before they can react with contaminants. Apply in the evening, early morning, or after rinsing the turf with water to bring the surface temperature down. This is the single most impactful variable in product performance.

✅ Rinse the area first before applying BioS+. A pre-application rinse flushes loose surface waste, dilutes urine concentration in the upper fiber layer, and activates the enzyme formula once applied. Skipping the pre-rinse reduces how deeply the enzymes penetrate into the fiber bed.

✅ BioS+ and BioX are different products for different purposes. BioS+ is an odor eliminator and enzyme cleaner. BioX is a disinfectant that kills bacteria and pathogens. For routine pet odor control, use BioS+. For disinfection after illness, parvo exposure, or commercial facility cleaning, use BioX. Do not mix both in the same bottle.

 

How Much TurFresh BioS+ Do You Need?

Before mixing, calculate how much product your yard requires. One 32 oz bottle of ready-to-use BioS+ solution covers approximately 500 square feet of artificial grass.

Small yard (up to 500 sqft): one 32 oz bottle per application session.

Medium yard (500 to 1,000 sqft): two 32 oz bottles or one 16 oz Super Concentrate mixed into two bottles.

Large yard (1,000 to 2,000 sqft): one 16 oz Super Concentrate mixed across four 32 oz bottles, or one gallon of ready-to-use solution.

Designated pet zones only (200 to 300 sqft): one 16 oz Super Concentrate typically lasts 4 to 6 weeks with twice-weekly applications.

For multi-dog households where frequency is high, the case pack options are more cost-effective than individual bottles. The BioSiphon sprayer connects directly to the 32 oz concentrate bottle and mixes at a 30:1 ratio automatically through the hose, covering large areas without manual mixing.

 

TurFresh BioS+ Mix Ratio: How to Mix the Concentrate Correctly

The BioS+ Super Concentrate must be diluted before use. The correct ratio produces a solution with the right enzyme concentration for effective odor control.

Standard mix ratio for a 32 oz bottle:
3.2 oz of BioS+ Super Concentrate + 28.2 oz of water = 32 oz ready-to-use solution.

Standard mix ratio for a half-gallon:
6.4 oz of BioS+ Super Concentrate + 57 oz of water = 64 oz ready-to-use solution.

Standard mix ratio for a gallon:
16 oz of BioS+ Super Concentrate + 112 oz of water = 128 oz ready-to-use solution.

Mixing instructions: Pour the concentrate into the bottle first, then add water. Shake thoroughly before use. Apply immediately after mixing. Do not mix in advance and store the diluted solution as enzyme activity diminishes within hours of dilution.

Adjusting concentration for heavy contamination

For areas with severe urine buildup or heat-activated odors, the concentration can be increased moderately to 4 oz concentrate per 28 oz water. Do not significantly exceed this ratio. Higher concentration does not proportionally increase effectiveness and depletes product faster than necessary. For contamination that has reached the infill layer, professional cleaning is more effective than any surface application regardless of concentration.

📌 The BioSiphon sprayer attached to your garden hose automatically mixes BioS+ at a 30:1 ratio as water flows through the bottle. This delivers consistent dilution across large areas without manual measuring and is the recommended application method for full-yard treatment.

 

When to Apply TurFresh Enzyme Cleaner for Best Results

Timing is the variable that most affects how well BioS+ performs. Enzymes are biological catalysts sensitive to temperature in ways that chemical cleaners are not.

Best time of day: apply in the evening after the sun has moved off the turf surface, or in the early morning before temperatures rise. Afternoon application in direct sunlight significantly reduces enzyme effectiveness.

Surface temperature: if the turf is hot to the touch, rinse the area thoroughly first. Allow 5 to 10 minutes for the surface to cool before applying BioS+. A cool, damp surface is the ideal condition for enzyme activity.

After rain or rinsing: applying BioS+ immediately after rain or a routine rinse is effective because the surface is cool and the pre-rinse has already flushed loose surface waste.

Seasonal note: in hot climates where turf surfaces can exceed 150 degrees Fahrenheit in afternoon sun, evening application is not just preferred but necessary for the product to work correctly.

 

How to Apply TurFresh BioS+: Step by Step

Step 1: Remove solid waste

Remove all solid pet waste from the surface before any application. Enzymes applied over solid organic material react with the waste mass rather than penetrating to the fiber and infill where uric acid crystals accumulate.

Step 2: Pre-rinse the area

Rinse the affected area thoroughly with a garden hose. This flushes loose urine residue through the drainage layer, cools the surface, and creates the damp surface conditions that allow enzyme penetration. Allow the area to drain but remain damp before proceeding.

Step 3: Apply BioS+

Apply BioS+ evenly to the affected area using the BioSiphon sprayer or a pump sprayer for targeted applications. Saturate the surface so the solution reaches into the fiber bed. For high-use pet zones, apply generously enough that you can see the solution wetting the infill between the fibers.

Step 4: Allow full dwell time

Allow a minimum of 10 minutes of dwell time. Do not rinse, walk on, or allow pets to use the treated area during this period. For heavily contaminated areas, 15 to 20 minutes produces better results. Set a timer.

Step 5: Final rinse

After full dwell time, rinse the area thoroughly to flush broken-down waste and residual solution through the drainage layer. The surface is ready for pet use once it has drained and dried, typically 30 to 60 minutes after the final rinse.

 

BioS+ vs BioX: Which TurFresh Product Do You Need?

TurFresh BioS+ is an enzyme cleaner and odor eliminator. It uses bacterial enzyme technology to break down uric acid, ammonia, and the organic compounds that cause pet odor. It does not disinfect. Use BioS+ for routine pet odor control and weekly maintenance of pet zones.

TurFresh BioX is a disinfectant formulated to kill bacteria, viruses, and pathogens on the turf surface. Use BioX when disinfection is the priority: after a pet illness, confirmed pathogen exposure such as parvo, or for commercial facilities with high dog density.

Combined protocol for high-use households: apply BioS+ first for odor control with full dwell time and rinse, then apply BioX for disinfection with its own dwell time, followed by a final rinse. Do not mix the two products in the same bottle as this reduces the effectiveness of both.

 

Indoor vs Outdoor Application

Outdoor turf: use the BioSiphon hose-end sprayer for full coverage. The hose provides the water volume needed to penetrate the fiber bed and carry the solution through to the drainage layer.

Indoor turf: most indoor installations lack drainage. Use a pump sprayer to apply BioS+ to targeted spots rather than saturating the full surface. Apply conservatively to avoid over-saturating a surface that cannot drain. Avoid the BioSiphon hose attachment for indoor use as the water volume is too high for enclosed spaces without drainage.

 

When BioS+ Is Not Enough

BioS+ is highly effective for surface-level odor control and routine pet zone maintenance. When uric acid crystals have accumulated in the lower infill layer over months of heavy pet use, the odor source is below the reach of any surface application.

The signal that infill saturation has occurred: odor returns within 24 to 48 hours of a correct BioS+ application with full dwell time. If this happens consistently, professional cleaning with extraction equipment that reaches the infill layer is the appropriate next step.

 

 

Frequently Asked Questions

 

What is the correct mix ratio for TurFresh BioS+ Super Concentrate?

Mix 3.2 oz of BioS+ Super Concentrate with 28.2 oz of water to produce one 32 oz ready-to-use bottle. For a half-gallon, use 6.4 oz concentrate with 57 oz water. For a gallon, use 16 oz concentrate with 112 oz water. Mix only what you plan to use immediately as diluted enzyme solution loses effectiveness within hours.

How long should TurFresh BioS+ sit before rinsing?

Allow a minimum of 10 minutes of dwell time before rinsing or allowing foot traffic. For heavily contaminated areas, 15 to 20 minutes produces better results. Rinsing immediately after application stops the enzymatic process before it can break down uric acid and organic compounds.

Why should you not apply BioS+ in direct sunlight?

Heat deactivates enzyme formulas before they can complete the odor breakdown process. Applying to hot turf in direct sun significantly reduces effectiveness. Apply in the evening, early morning, or after rinsing the turf with water to cool the surface first.

How much BioS+ do you need for your yard?

One 32 oz bottle of ready-to-use BioS+ covers approximately 500 square feet. A small yard under 500 sqft needs one bottle per application. A medium yard of 500 to 1,000 sqft needs two bottles or one 16 oz Super Concentrate mixed across two bottles.

What is the difference between TurFresh BioS+ and BioX?

BioS+ is an enzyme cleaner and odor eliminator that breaks down uric acid and organic waste. BioX is a disinfectant that kills bacteria and pathogens. Use BioS+ for routine odor control. Use BioX when disinfection is needed, such as after pet illness or for commercial facilities. Do not mix both products in the same bottle.

Can TurFresh BioS+ be used indoors?

Yes, but with a pump sprayer rather than the BioSiphon hose attachment. Indoor turf typically lacks the drainage that outdoor installations have, so apply conservatively to targeted spots and avoid over-saturating a surface that cannot drain.

Why does pet odor come back after using BioS+?

If odor returns within 24 to 48 hours of a correct application with full dwell time, the odor source has reached the infill layer below the surface where any topical application stops. Professional cleaning with extraction equipment is the appropriate solution at this stage.