TurFresh Enzyme Cleaner: How BioS+ Works and When to Use It

Quick Answer:
TurFresh enzyme cleaner BioS+ uses a bio-enzymatic formula, water, viable bacterial culture blend, non-ionic surfactant, anionic surfactant, and fragrance, to break down uric acid, ammonia, and bacteria in artificial turf at the source rather than masking them. Each quart covers up to 500 square feet. For routine maintenance and mild to moderate pet use, BioS+ applied with correct dwell time resolves urine odor completely. When odor returns within 72 hours of a correct application, the infill is saturated beyond what any at-home product can reach.

 

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BioS+ handles maintenance. When the infill is saturated, we handle the rest.

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Key Takeaways

✅ BioS+ is a bio-enzymatic formula, not a fragrance spray. It contains a viable bacterial culture blend that consumes uric acid, ammonia, and organic waste at a molecular level. The scent is a byproduct of the process, not the mechanism.

✅ Dwell time is the step most homeowners skip. Enzymes need 10 to 15 minutes of contact with the surface to complete the breakdown cycle. Rinsing too soon stops the process and leaves odor-causing compounds behind.

✅ Coverage is 500 square feet per quart. A one-gallon jug treats 4,000 to 5,000 square feet depending on pet use intensity. Heavy-use zones may require more frequent applications or higher volume.

✅ Apply in cooler conditions. Direct sunlight and high surface temperatures cause the bacterial culture to dry out before completing the dwell cycle. Early morning or evening application is consistently more effective in hot climates.

✅ If odor returns within 72 hours, the problem is in the infill. BioS+ is designed for surface and fiber cleaning. Uric acid crystals embedded deep in compressed infill require professional extraction equipment to remove.

 

What Is TurFresh BioS+ and How Does It Work?

TurFresh BioS+ is a bio-enzymatic turf cleaner formulated specifically for artificial grass systems. Unlike surface sprays or scented deodorizers, it works through two simultaneous biological processes.

The first is enzymatic action. The formula contains enzymes that target and break down the specific organic compounds found in pet waste: uric acid crystals, urea, ammonia, and the proteins that feed odor-producing bacteria. These enzymes work at a molecular level, not at the surface level.

The second is bioaugmentation. The viable bacterial culture blend in the formula introduces beneficial microorganisms that consume the broken-down waste before it can regenerate. This is why BioS+ produces longer-lasting results than products that use disinfectants or fragrances: after application, the biological process continues working for hours.

The complete ingredient list, as listed on the product: water, viable bacterial culture blend, non-ionic surfactant, anionic surfactant, and fragrance. The unscented formula omits the fragrance component. There are no GMO organisms, harsh chemicals, or synthetic disinfectants.

📌 The non-ionic and anionic surfactants in the formula act as penetrating carriers, helping the enzyme solution move through turf fibers and into the upper infill layer where odor compounds accumulate.

 

How to Apply TurFresh BioS+ Correctly

The application protocol determines whether BioS+ resolves the odor or produces only a temporary improvement. Most cases where the product “did not work” involve a skipped or shortened step.

Step 1: Pre-rinse the surface

Rinse the affected area with water before applying the product. This removes loose debris, dilutes surface urine, and pre-moistens the turf fibers. Moist fibers allow the enzyme solution to penetrate more effectively than dry ones.

Step 2: Apply BioS+ evenly

Attach the BioSiphon sprayer to your garden hose for even coverage. Spray the affected area and any surrounding high-use zones. Each quart covers up to 500 square feet. For a heavily saturated zone, apply more generously rather than diluting coverage.

Step 3: Respect the dwell time

Allow the solution to remain on the surface for a minimum of 10 to 15 minutes. This is the most critical step. The enzymes require contact time to break down uric acid and ammonia compounds. Do not rinse immediately.

Step 4: Rinse thoroughly

After the full dwell time, rinse the entire treated area from one end to the other. This flushes the broken-down waste through the turf and infill drainage system.

Step 5: Apply in cool conditions

For best results, apply BioS+ in the early morning or evening, not in direct afternoon sun. In hot climates such as Phoenix, Las Vegas, and the Inland Empire, surface temperatures can exceed 130 degrees Fahrenheit in summer. High heat causes the bacterial culture to dry and denature before completing the dwell cycle. A product applied at 7 AM will consistently outperform the same product applied at 2 PM.

📌 According to TurFresh product documentation, the BioSiphon sprayer connects directly to a standard garden hose and is calibrated for optimal coverage per square foot. Using a standard spray bottle on a large yard results in uneven distribution and higher product consumption.

 

How Much BioS+ Do You Need?

Coverage depends on yard size and pet use intensity.

32 oz bottle: covers up to 500 square feet under standard pet use. Good for a small yard or targeted spot treatment.

1-gallon jug: covers 4,000 to 5,000 square feet. Appropriate for average residential yards with one or two dogs.

5-gallon bucket: designed for large residential properties, kennels, dog runs, and commercial installations with high pet volume.

For households with multiple dogs in a small yard, treat the entire turf surface as a high-use zone and apply monthly or more frequently depending on odor recurrence. First applications on heavily saturated turf typically require more product volume to reach accumulated odor compounds than subsequent maintenance applications.

 

Is TurFresh BioS+ Safe for Pets, Kids, and the Environment?

Yes. BioS+ is formulated with naturally occurring bacteria and contains no GMO organisms, harsh chemicals, or synthetic disinfectants. The formula is non-corrosive, non-toxic, and biodegradable.

Pets and children can use the treated area immediately after application. There is no required wait period for re-entry.

The formula is also safe for surrounding landscaping, concrete, and drainage systems. Beyond artificial turf, BioS+ can be used on any outdoor surface where organic odors accumulate: concrete patios, kennels, gravel runs, outdoor carpet, and equipment storage areas.

📌 TurFresh BioS+ contains no genetically modified organisms. This is confirmed by TurFresh on all product pages and relevant for households with organic-certified landscaping adjacent to the turf area.

 

BioS+ vs. BioX: Which One Do You Need?

Both products eliminate turf odors, but they work through different mechanisms and perform better under different conditions.

TurFresh BioS+ is enzymatic and biological. It works by introducing live bacterial cultures that consume organic waste over time. It is the right choice for routine maintenance, mild to moderate pet use, and climates where dwell time can be completed without premature drying.

TurFresh BioX is oxidant-based. It does not rely on live bacterial activity, which means temperature and drying conditions do not affect its performance in the same way. It is the better choice for persistent or recurring odors, heavily saturated infill, and high-heat climates where enzyme-based products struggle to complete their dwell cycle consistently.

If you have one or two dogs and are starting a maintenance routine, BioS+ is the correct product. If you have multiple dogs, if odor keeps returning despite consistent BioS+ applications, or if you are in Phoenix, Las Vegas, or another high-heat market and applying at midday, BioX will deliver more consistent results.

📌 The choice between BioS+ and BioX is not about which product is stronger. It is about which mechanism matches your conditions. Enzymatic cleaners and oxidant cleaners work in fundamentally different ways.

 

Does TurFresh BioS+ Remove Pet Odors Permanently?

Yes, when applied correctly and maintained consistently. The bacterial culture eliminates the compounds that produce odor rather than covering them. With a proper maintenance routine, BioS+ prevents odor accumulation from reaching the threshold where it becomes noticeable.

What BioS+ cannot do is penetrate deep into compacted, saturated infill where years of uric acid buildup have accumulated below the reach of any topical application. In this case, the product delivers temporary relief but odor returns within 24 to 72 hours. This is not a product failure. It is a signal that the infill requires mechanical extraction, which only professional cleaning equipment can achieve.

Maintenance schedule by use level:

One dog, average use: rinse weekly, apply BioS+ monthly, professional cleaning once or twice per year.

Two or more dogs: rinse 2 to 3 times per week, apply BioS+ every 2 to 3 weeks, professional cleaning every 3 months.

Kennels, dog runs, commercial use: apply BioS+ weekly, professional cleaning monthly or as volume requires.

 

When BioS+ Is Not Enough

BioS+ is designed for maintenance and odor prevention. There are two situations where it reaches its functional limit.

The first is deep infill saturation. Uric acid crystals are hydrophobic: they resist water and are not easily displaced by topical application. When multiple years of pet use have compressed them into the lower infill layer, no liquid product applied from the surface can fully reach or neutralize them. The odor returns consistently and quickly because the source is below the product's penetration depth.

The second is drainage failure. If standing water or persistent moisture remains in the turf system after rain or irrigation, odor-producing bacteria have ideal conditions to multiply faster than any maintenance product can control. This is a structural issue, not a cleaning issue.

In both cases, professional deep cleaning with commercial-grade extraction equipment is the reliable solution. TurFresh technicians use equipment that reaches the infill and backing layers, removes compacted material, and treats the full turf system rather than the surface alone.

📌 If turf odor returns within 72 hours of a correct BioS+ application, the infill has accumulated beyond what topical maintenance can address. This is the clearest signal that professional service is the next step.

 

BioS+ is not cutting it anymore?

When the problem is in the infill, you need professional equipment to solve it.

TurFresh deep-cleans turf fibers, infill, and drainage layers with commercial-grade equipment. Over 150,000 services completed across California, Arizona, Texas, Nevada, and Florida. Our 30-day odor removal guarantee means you get results or we come back.

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Frequently Asked Questions

 

What is TurFresh enzyme cleaner BioS+?

TurFresh BioS+ is a bio-enzymatic artificial turf cleaner that eliminates pet odors by breaking down uric acid, ammonia, and bacteria at a molecular level. It contains a viable bacterial culture blend that continues working after application, producing longer-lasting results than fragrance-based or disinfectant-based alternatives.

How often should I apply TurFresh BioS+?

For one dog with average use, monthly application is sufficient for maintenance. For two or more dogs, apply every 2 to 3 weeks. For kennels or commercial use, weekly application is recommended. Increase frequency during warm months when bacterial activity is higher.

How much BioS+ do I need for my yard?

Each 32 oz bottle covers up to 500 square feet. A one-gallon jug covers 4,000 to 5,000 square feet. For large yards or properties with heavy pet use, the 5-gallon bucket provides the best cost per square foot.

Why does the smell come back after using BioS+?

If odor returns within 72 hours of a correct application with full dwell time, the uric acid buildup is deep in the infill layer, beyond the reach of any topical product. This is the signal that professional deep cleaning is needed. If odor returns after several days, increase application frequency or extend dwell time.

Is TurFresh BioS+ safe for dogs and kids?

Yes. The formula uses naturally occurring bacteria with no GMO organisms, harsh chemicals, or synthetic disinfectants. It is non-toxic, non-corrosive, and biodegradable. Pets and children can use the area immediately after application with no required wait period.

When should I use BioX instead of BioS+?

Use TurFresh BioX when odor is persistent despite consistent BioS+ applications, when you have multiple dogs, or when your climate makes enzyme dwell time difficult to complete. BioX is oxidant-based and not dependent on live bacterial activity, making it more consistent in high-heat conditions like Phoenix and Las Vegas.

Can BioS+ be used on surfaces other than artificial turf?

Yes. BioS+ works on any outdoor surface where organic odors accumulate: concrete patios, gravel dog runs, kennels, outdoor carpet, and decking. The formula is safe for all these surfaces and surrounding landscaping.

 

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John Pla is the owner of TurFresh and an expert with over 20 years of experience in artificial turf cleaning and maintenance. John’s passion for sustainability, community impact, and innovative solutions has made him a trusted figure in the artificial grass industry and beyond.